Toluidine Blue – Pointer – CAS 6586-04-5 – Biological Stain and Indicator Dye

Toluidine Blue, also known as Pointer Blue (CAS 6586-04-5), is a basic thiazine metachromatic dye commonly used as a biological stain, histology reagent, and chemical indicator. It binds to acidic tissue components like nucleic acids, making it especially valuable for staining mucopolysaccharides, cartilage, and mast cell granules.

With its intense blue-violet color and metachromatic properties, Toluidine Blue is widely used in research, pathology labs, microbiology, and material analysis.

🧪 Common Applications:

• Staining of biological tissues (RNA, DNA, cartilage)

• Histology and cytology specimen preparation

• Indicator in pH and chemical tests

• Plant and fungal cell analysis

• Educational microscopy and lab demonstrations

Specifications:

• Chemical Name: Toluidine Blue

• Synonym: Pointer Blue

• CAS Number: 6586-04-5

• Appearance: Dark blue or violet powder

• Solubility: Soluble in water and ethanol

• Packaging: Light-protective, sealed laboratory container

Safety & Storage:

• Avoid inhalation and direct contact with skin or eyes

• Use standard lab PPE: gloves, eye protection, lab coat

• Store in a dry, cool place, away from direct sunlight and oxidizers

GHS Hazard Classification:

• Signal Word: Warning

• Hazard Statements:

  • H302 – Harmful if swallowed

  • H319 – Causes serious eye irritation

• Precautionary Statements:

  • P280 – Wear protective gloves/eye protection

  • P264 – Wash hands thoroughly after handling

  • P301 + P312 – If swallowed, seek medical advice